Sequencing Request Form

Discussion

The Sequencing Request Form is used to capture the sequencing requests and enter them into the system.

The form prompts the user for how many sequencing requests will be entered and based upon that number, dnaLIMS dynamically creates a table of requests with that number of rows.

The form requires the user to enter a Sample Name then select attributes associated with the sample.

After the Sequencing Request Form is filled in, the user selects the Validate button to test if all values within the form are filled in.

The Submit button is selected to enter the request into the sequencing request queue. After the requests are accepted, a summary table of the requests is displayed. Please print two copies of this form and bring one of the forms to the core lab with your samples.

Sample Name

Each sequencing request is required to have a sample name. The sample name has specific restrictions,
the name cannot be longer than 20 characters and can only contain legal characters.
Legal characters are: a-z, A-Z, 0-9, and -
Illegal characters are any other characters.
Do not use underscore or period.

Index Samples

This feature allows the user to append a range of numbers to the sample name:

sample1, sample2, sample3....
or
sample7, sample8, sample9....

The user can create up to 96 individual sample requests with this feature.

To use this feature:

1. Enter a Sample Name in the first row of the table.
2. Select the Index icon within the Sample Name column.
3. Enter the beginning number within the dialog box.

Alternate Primers

This feature allows the user to toggle/alternate between two primers, i.e.

sample1 primer1
sample1 primer2

sample2 primer1
sample2 primer2

The Alternate Primers feature is used in conjunction with the Index Sample Name feature to provide a range of sample names with two different primers.

The user can create up to 48 unique sample names with two different primers per sample name.
This creates a total of 96 sample requests.

To use this feature:

1. Enter a Sample Name in the first row of the table.
2. Select the Fill 2X icon and enter the beginning number within the dialog box
3. Select the first primer with the Primer select list in the first row.
4. Select the second primer with the Primer select list in the second row.
5. Select the Alternate icon within the Primer column.

Primer (select list and text box)

Each sequencing request is required to have a primer. A system primer or a custom primer can be selected. Select either a system primer or a custom primer with the Primer select list. After a system primer is selected the primer name will fill the Primer Name text box. If Custom is selected in the Primer select list, a dialog box will appear for user entry of the primer name. Create the custom primer before using the custom primer name.

Do not manually edit the name in the Primer Name text box.

Legal characters for primer names are: a-z, A-Z, 0-9 and -
Do not use underscore or period.

Summary Table

Print two copies of the summary table and bring one with the samples to the core facility. Keep the other for your records.